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HelpTest Code UIEX Protein Electrophoresis with Immunofixation, Urine, 24 hour
Specimen and Container/Tube
Urine, Timed (24 hour)
Rejection Criteria
Do not use boric acid, acetic acid or hydrochloric acid as a
preservative.
Turnaround Time
N/A
Test Includes
Total Protein, Protein Electrophoresis, Immunofixation
Electrophoresis with urine concentration for IgG, IgA, IgM,
Kappa
and Lambda.
Specimen Type
Urine, Timed (24 hour)
Container/Tube
Sterile Urine Container
Preferred Volume
10 mL
Specimen Minimum Volume
5 mL
Reject Due To
Do not use boric acid, acetic acid or hydrochloric acid as a
preservative.
Day(s) Performed
Monday – Friday
STAT DAY(S) AND TIME(S) PERFORMED
N/A
STAT Turnaround Time
N/A
Method Name
Agarose Gel Electrophoresis & Immunofixation
Synonyms
N/A
Test Limitations
Fresh 24 hour urine or first morning catch is preferred to
avoid
possible interferences such as contamination with bacteria,
increase in pH due to breakdown of urea to ammonia or
degradation
of proteins by proteases.
Test Usage
Urine protein electrophoresis may help identify monoclonal
proteins which are excreted into the urine and may elude
detection
by serum protein electrophoresis, especially the Bence-Jones
Proteins (monoclonal free kappa or lambda light chains) which
are
characteristic of light chain disease.
Offsite Collection Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ° C).
UCMC Collection Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ° C).
Reference Values
No monoclonal proteins detected.
Interpretive report provided by pathologist.
Additional Information
N/A
Stability: Room Temp
2 hours
Stability: Refrigerated
7 days
Stability: Frozen
1 month
Pediatric Volume
5 mL
Clinical Indications
Urine protein electrophoresis may help identify monoclonal
proteins which are excreted into the urine and may elude
detection
by serum protein electrophoresis, especially the Bence-Jones
Proteins (monoclonal free kappa or lambda light chains) which
are
characteristic of light chain disease.
Transport Instructions
1. Follow clinic instructions for 24 hour collection
process.
2. Keep urine refrigerated during the 24 hour collection
process.
3. Aliquot 10 mL from a well mixed timed sample collection.
4. Indicate collection volume and duration.
5. Transport refrigerated (2-8 ?� C).
Test Components
Total Protein, Protein Electrophoresis, Immunofixation
Electrophoresis with urine concentration for IgG, IgA, IgM,
Kappa
and Lambda.
Specimen Stability
2 hours
Methodology
Agarose Gel Electrophoresis & Immunofixation
Synonyms/Key Search Words
N/A