Sign in →

Test Code TETF Testosterone, Total & Free

Specimen Type

Serum

Container/Tube

Red Top

Offsite Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be spun and aliquoted
3. Transport refrigerated  (2 – 8C).

Turnaround Time

4 - 5 days

Test Includes

Total Testosterone, Sex Hormone Binding Globulin, Albumin, Calculated Free Testoserone

Preferred Volume

1.0 mL

STAT Turnaround Time

N/A

Critical Results

N/A

Fee Code

43259, 43218, 43372

Specimen Minimum Volume

0.5 mL

DAY(S) AND TIME(S) PERFORMED

Tuesday & Friday

STAT DAY(S) AND TIME(S) PERFORMED

N/A

CPT

84403, 84270, 82040

Method Name

Spectrophotometric, Liquid Chromatography / Tandem Mass Spectometry (LC-MS/MS)

Reference Values

Refer to total testosterone, albumin and SHBG tests for reference range information.
 

Free Testosterone
Males:
Prepubertal (5-9 years) <3.0* pg/mL
Age 17-55 years 90-300 pg/mL [or 9.0-30.0 ng/dL]
 

Females:
Prepubertal (5-8 years) <3.0* pg/mL
Age 11-40 years 3.0-9.0* pg/mL [or 0.3-0.9 ng/dL]

 

*Derived by calculation from our normative data established in carefully screened children (6-11 yrs old) and young women(11-40 yrs old, eumenorrheic, nonhirsute, with ultrasonographically normal ovaries, reference attached), we expect the normal upper limit (95th percentile) of free testosterone to be <3.0 pg/ml in prepubertal children and 9.0 pg/mL in women. For pubertal children, free testosterone values are intermediate and fall between prepubertal and adults values and overlap with both. For aging men and post-menopausal women, values fall proportionally as much as or more than total testosterone.

 

References
1. Children: Bordini BD, Littlejohn EE, Rosenfeld RL. J Clin Endocrinol Metab 2009; 94: 1168-1175
2. Women: Mortensen M, Ehrmann DA, Littlejohn E, Rosenfield RL. J Clin Endocrinol Metab 2009; 94: 1579-86
3. Rosenfield RL, Mortensen M, Wroblewski K, Littlejohn E, Ehrmann DA. Determination of the source of androgen excess in functionally atypical polycystic ovary syndrome by a short dexamethasone androgen-suppression test and a low-dose ACTH test. Hum Reprod 2011;26:3138-46
4. Ober C, Loisel DA, Gilad Y. Sex-specific architecture of human disease. Nat Rev Gen 2008;doi:10.1038/nrg2415.

Reject Due To

Whole blood/unspun samples greater than 6 hours will be rejected.

Synonyms

N/A

Test Limitations

See specific analytes for testing limitations.

Test Usage

N/A

UCMC Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be received in Laboratory Service Center
3. Transport room temperature (15 – 25C).

Additional Information

N/A

Included in Additional codes

N/A

Coverage Information

N/A

Stability: Room Temp

See individual analytes for stability information