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Mayo Clinic Laboratories

Test Code RTCVGP Blood Gas, Venous, Cord Blood 

Specimen and Container/Tube

Specimen Type: Umbilical cord blood

Preferred Specimen: Syringe

Other Acceptable Specimens: None

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

30 minutes

Refrigerated

not acceptable

Frozen

not acceptable

Collection Instructions

  • Collect specimen per standard laboratory collection procedures. 
  • Remove all air from syringe. 
  • Remove needle. 
  • Cap tightly and mix well. 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

  • Collect specimen per standard collection procedure and send to laboratory immediately. 
  • Testing must be performed within 30 minutes of collection.
  • Do NOT send on ice. 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Specimens are not acceptable from offsite locations for blood gas testing 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens with needles attached will be rejected. 
  • Specimens with air bubbles.
  • Cord blood is considered an irreplaceable body fluid. If a misidentified cord blood is received in the laboratory, the staff will call the point of origin and require that the individual responsible for the specimen collection/specimen labeling (or, in their absence, the ordering or other responsible physician) come to the laboratory and complete the following:
  • Misidentified cord blood gas specimens will be analyzed upon receipt, but results will not be released until the proper identification process has been completed.

Reference Values

Analyte

Age

Sex

Normal Range

pH, venous cord

All

All

7.22 – 7.44

pCO2, venous cord

All

All

30 – 63 mmHg

pO2, venous cord

All

All

12 – 43 mmHg

HCO3, venous cord

All

All

20 – 28 mmol/L

Base excess, venous cord

All

All

-6.0 – 2.0 mmol/L

SO2 calculated, venous cord

All

All

23 – 100%

SVO2, venous cord

All

All

23 – 100%

Hemoglobin, venous cord

All

All

11.4 – 19.0 g/dL

Oxyhemoglobin, venous cord

All

All

23.0 – 94.0%

Carboxyhemoglobin, venous cord

All

All

0.2 – 7.3%

Methemoglobin, venous cord

All

All

0.5 – 1.4%

Deoxyhemoglobin, venous cord

All

All

0.0 – 76.0%

Critical Values

Analyte

Age

Low

High

pH, venous cord

All

<7.00

NA

Base excess, venous cord

All

<-12 mmol/L

NA

Hemoglobin, venous cord

All

<6.0 mg/dL

NA

Carboxyhemoglobin, venous cord

All

NA

>20.0%

Methemoglobin, venous cord

All

NA

>10.0%

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Test Components

pH, PCO2, PO2, HCO3-, Base Excess, SO2, SVO2, Hemoglobin, Oxyhemoglobin, Deoxyhemoglobin, Carboxyhemoglobin, Methemoglobin

Methodology

Potentiometry: pH, pCO2

Amperometry: pO2

Derived: HCO3-, base excess

Optical (CO-oximetry): SO2, hemoglobin, oxyhemoglobin, deoxyhemoglobin, carboxyhemoglobin, methemoglobin

CPT Codes

82805

LOINC Codes

TBD

Additional Information

  • For SO2: Measured oxygen saturation of functional hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb).
  • For OxyHb: Measured oxygen saturation of total hemoglobin according to the following formula. OxyHb/(OxyHb + DeoxyHb + COHb + MetHb).

Last Review Date

4/29/2025