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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code RF NEW Rheumatoid Factor

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Serum/Gold Top, Serum/Red Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

  • Samples should be centrifuged within 2 hours of collection.
  • Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
  • Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
  • Samples must be sent room temperature at 15-25oC.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that are clotted or grossly hemolyzed.
  • Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

 

Test

Age

Sex

Reference Range

Rheumatoid Factors

All

All

≤ 14 IU/mL
       
       

Critical Values

Not applicable

CPT Codes

86431

LOINC Codes

TBD

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

24 hours

Refrigerated

3 days

Frozen

4 weeks 

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Test Components

Rheumatoid Factors

Clinical Indications

Measurements may be used as an aid in the diagnosis of rheumatoid arthritis.

Methodology

Immunoturbidimetric Assay (Roche Diagnostics)

Additional Information

Rheumatoid factors are also found in other inflammatory rheumatic diseases and in various non-rheumatic diseases. They are also found in clinically healthy persons over 60 years of age.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: 1 hour 

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Synonyms/Keyword Search

RF

Last Review Date

4/18/2025