Urine Amylase, Random - UChicago Medicine Medical Laboratories

Specimen and Container/Tube

Urine, random

Rejection Criteria

Urine containing chelating agents (citrates, oxalates,
EDTA) must not
be used.

Turnaround Time

N/A

Test Includes

Quantitative determination of amylase in urine

Specimen Type

Urine, random

Container/Tube

Sterile Urine Container

Preferred Volume

0.5 mL

Specimen Minimum Volume

0.2 mL

Reject Due To

Urine containing chelating agents (citrates, oxalates,
EDTA) must not be used.

Day(s) Performed

Monday – Friday

STAT DAY(S) AND TIME(S) PERFORMED

N/A

STAT Turnaround Time

N/A

Method Name

Enzymatic Colorimetric Assay
Spectrophotometric

Synonyms

N/A

Test Limitations

a- Amylase is unstable in acid urine. Assay promptly or
adjust
pH to alkaline range (about pH 7) before storage. Freezing has
caused substantial loss of amylase activity in some urine
specimens
and is therefore not recommended.

Test Usage

Assist in the assessment of liver synthetic function.
Useful in the diagnosis and management of pancreatitis. An aid in
the evaluation of pancreatic function.

Offsite Collection Instructions

1. Collect without additives or preservatives.
2. Random urine collections only.
3. Transport refrigerated (2-8° C).

UCMC Collection Instructions

1. Collect without additives or preservatives.
2. Random urine collections only.
3. Transport refrigerated (2-8° C).

Reference Values

Reference ranges for random urine specimens have not been
established; subject to ordering physician interpretation only.

Additional Information

N/A

Stability: Room Temp

2 days

Stability: Refrigerated

10 days

Stability: Frozen

n/a

Pediatric Volume