Test Code QTBG QuantiFERON-TB Gold Plus

Specimen and Container/Tube

Whole Blood

Specimen Type

Whole Blood

Preferred Volume

4mL total ( 1mL per tube)

UCMC Collection Instructions

1. Collect 1 mL of blood by venipuncture directly into each of the QFT-Plus Blood Collection Tubes.

2. Tube may fill slowly. Hold tube for 2-3 seconds after flow ceases.

3. If a “butterfly needle” is being used to collect blood, a “purge” tube should be used to ensure that the tubing is filled with blood prior to the QFT-Plus Collection Tubes used.

4. Immediately after filling the tubes, shake them ten (10) times enough to make sure the entire inner surface of the tube is coated with blood.

5.Store at room temperature. DO NOT refrigerate.

6. Send to Laboratory Service Center (LSC) tube station #201 as soon as possible, or within 4 hours from collection time.

Offsite Collection Instructions

1. Collect 1 mL of blood by venipuncture directly into each of the QFT-Plus Blood Collection Tubes.

2. Tube may fill slowly. Hold tube for 2-3 seconds after flow ceases.

3. If a “butterfly needle” is being used to collect blood, a “purge” tube should be used to ensure that the tubing
is filled with blood prior to the QFT-Plus Collection Tubes used.

4. Immediately after filling the tubes, shake them ten (10) times enough to make sure the entire inner surface of the tube is coated with blood.

5.Store at room temperature. DO NOT refrigerate.

6. Specimens must arrive at UCMC Laboratories as soon as possible, or within 10 hours from collection time.

Container/Tube

Four special tubes (Gray cap with white ring, Green cap with white ring, Yellow cap with white ring, and Purple cap with white ring)

Specimen Minimum Volume

4mL total (1mL per tube)

Day(s) Performed

Monday through Friday

STAT DAY(S) AND TIME(S) PERFORMED

Not available

CPT

86480

Method Name

Enzyme-linked Immunosorbent assay (ELISA)

Reference Values

Negative

Pediatric Volume

4mL total (1mL per tube)

Transport Instructions

?�1. Collect 1 mL of blood by venipuncture directly into
each of the QFT-Plus Blood Collection
Tubes.

2. Tube may fill slowly. Hold tube for 2-3 seconds after
flow
ceases.

3. If a �butterfly needle�?� is being used to
collect
blood, a
�purge�?� tube should be used to ensure that the
tubing
is filled
with blood prior to the QFT-Plus
Collection Tubes used.

4. Immediately after filling the tubes, shake them ten (10)
times enough to make sure the entire inner surface of the tube
is
coated with blood.

5.Store at room temperature. DO NOT
refrigerate.

6. Specimens must arrive at UCMC
Laboratories as soon as
possible, or within 10 hours from collection time.

Methodology