Test Code PPGN Two Hour Post-Prandial Glucose Screen
Specimen and Container/Tube
Specimen Type: Plasma
Preferred Specimen: Grey Top, NaF/K-Oxalate
Other Acceptable specimens: None
Volume
Preferred Volume: 0.5 mL
Minimum Volume: 0.2 mL
Specimen Stability
Storage Temperature |
Stability |
Room temperature |
3 days |
Refrigerated |
3 days |
Frozen |
Not acceptable |
Collection Instructions
- Collect specimen per standard laboratory collection procedures.
- Fasting Draw: a fasting glucose sample is collected from the patient in a grey top (fluoride) vacutainer tube prior to Glucocrush® 75 ingestion. The sample is labeled PPG-Fasting.
-
Glucose Beverage Ingestion:
- Patient drinks the entire bottle of refrigerator-temperature Glucocrush® 75 within 5 minutes. Timing begins the moment the patient starts drinking.
- In the case of regurgitation, the test is discontinued unless the ordering physician can be contacted and decides to have the test continued. In this case, a note should be made on the requisition that the test has been performed in spite of the patient’s regurgitation, per doctor’s instruction.
- No smoking, gum-chewing, or eating during test. The patient may drink small volumes of water. The patient should remain seated as much of the time as possible.
- In some cases, the ordering physician will ask the patient to have a fasting sample drawn and then to ingest a specified meal and return for the 2-hour sample collection.
-
Specimen Collection
- A non-fasting sample is drawn into a grey Vacutainer tube 2 hours after finishing Glucocrush® ingestion. Sample is labeled PPG-NONFASTING, with indication of collection time.
- All blood samples are brought immediately to the lab for testing.
Specimen Handling and Transport Instructions
UCMC Onsite Instructions:
- Collect specimen per standard laboratory collection procedures.
UCMC Offsite and UC MedLab Outreach Instructions:
- Collect specimen per standard laboratory collection procedures.
- Centrifuge specimens at >2500 x g for 10 minutes at room temperature within 2 hours of collection.
- Aliquot removed plasma into a plastic tubes containing a minimum of 0.2 mL of specimen.
- Plasma samples must then be sent room temperature at 20 - 25oC
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Specimens that are clotted or grossly hemolyzed.
- Specimens that were transported unspun/whole blood and draw time is greater than 6 hours
Reference Values
Test |
Age |
Sex |
Reference Range |
Two hour post-prandial fasting glucose |
All |
All |
< 100 mg/dL |
Two hour post-prandial 2 hour glucose |
All |
All |
< 140 mg/dL |
Critical Values
Analyte |
Unit |
Low |
High |
Age |
Glucose |
mg/dL |
<40 |
>500 |
>14 days |
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: Not Available as STAT test
Routine Turnaround Time: 4 hours
Testing Schedule: 24/7
Clinical Indications
This is a screening test for possible diabetes mellitus. The test is so names because it was long ago common screening for diabetes mellitus by measuring blood glucose two hours following a meal. The test involves measuring plasma glucose from a sample taken before and two hours after a 75 gram oral dose of glucose. Alternatively, some physicians prescribe a specified meal in place of the oral glucose.
Test Components
Two hour post-prandial fasting glucose, two hour post-prandial two hour glucose
Methodology
Enzymatic Method with hexokinase (Roche Diagnostics)
CPT Codes
82947
LOINC Codes
TBD
Synonyms/Keyword Search
PPGN
Additional Information
- Results should always be assessed in conjunction with patient's medical history, clinical examination and other findings.
- Interpretation of Results:
- In a position statement of the American Diabetes Association (ADA) on the Diagnosis and Classification of Diabetes Mellitus in 2010 [Ref. 2], criteria for the provisional diagnosis of diabetes mellitus may be made when:
- Fasting plasma glucose level of ≥ 126 mg/dL OR
- A 2 hour post load glucose level of ≥ 200 mg/dL during an oral glucose tolerance test (using a 75 g glucose load) OR
- Any casual (random) glucose level or ≥200 mg/dL with symptoms of hyperglycemia or hyperglycemic crisis.
- Intermediate states indicative of impaired glucose homeostasis that do not meet the diagnostic criteria for diabetes mellitus include:
- Impaired fasting glucose (IFG), where fasting plasma glucose levels are 100-125 mg/dL
- Impaired glucose tolerance test (IGT), where 2 hour post 75 g glucose load glucose levels are 140-100 mg/dL during an oral glucose tolerance test.
Last Review Date
6/4/2025