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Test Code P2Y12P P2Y12 Inhibition Assay

Specimen and Container/Tube

Specimen Type: Whole Blood

Preferred Specimen: Light Blue Top Tube, 3.2% Sodium Citrate

Other Acceptable specimens: n/a

Volume

Preferred Volume: 2 Light Blue Top Tubes, 3.2% Sodium Citrate

 

Minimum Volume: 2 Light Blue Top Tubes, 3.2% Sodium Citrate

Specimen Stability

Storage Temperature

Stability

Room temperature

4 hours

Refrigerated

not acceptable

Frozen

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

 

Given the critical importance of
platelets remaining unactivated prior to being exposed to the
ADP stimulus in the course of this assay procedure,

it is vital that the venipuncture be smooth and the
blood flows readily. If these criteria are not achieved, the
tube should be discarded and another venipuncture
performed. It is also essential to follow the proper Order of
Draw. Immediately after collection please invert the sample
4-5 times gently; the tube must never be rapidly shaken.

 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: 

Collect specimen per standard collection procedure and send to laboratory immediately. 

 

UCMC Offsite and UC MedLab Outreach Instructions: 

Whole blood sample must arrive in the laboratory within
three hours of specimen collection.

Draw two 3 mL light blue top tubes. Invert gently, do not shake.

NEVER CENTRIFUGE. NEVER FREEZE.

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Specimens that have wrong ratio of blood to anticoagulant (fill volume), clotted or grossly hemolyzed.

Reference Values

 

Test

Age

Sex

Reference Range

Test component

Age range/phase

Male, Female, or all

Insert range

P2Y12

All

All

210 - 325 PRU

Critical Values

Analyte

Unit

Low

High

Age

n/a

 

 

 

 

Test Components

P2Y12 Inhibition

 

Clinical Indications

Measuring residual platelet function in patients on
P2Y12 inhibitors (clopidogrel, prasugrel, ticagrelor)

Methodology

Platelet aggregation

CPT Codes

85576 X2

LOINC Codes

53813-2

Additional Information

This test is intended to evaluate the degree of functional
platelet inhibition achieved following administration of P2Y12
receptor antagonist drugs. Whenever possible, it is strongly
recommended to perform this test prior to institution of such
therapy in order to provide a baseline from which achieved
percent inhibition of response can subsequently be ascertained after
the patient has begun receiving the antagonist drug.

Note: For platelet counts outside the range of 120,000/uL-500,000/uL,
nonspecific interference at the analytical testing level may
potentially produce misleading results with respect to P2Y12
receptor antagonism. Testing must be performed within 4 hours
after the sample is drawn.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: n/a

 

Routine Turnaround Time: 4 hours

 

Testing Schedule: 7 days a week, 5:00 am to 10:30 pm

Last Review Date

04/08/2022