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Test Code OBGTT Glucose Tolerance Test, Gestational, Plasma (Epic Code)

Specimen and Container/Tube

Plasma

Rejection Criteria

N/A

Test Includes

N/A

Specimen Type

Plasma

Container/Tube

Gray Top
Na-Fluoride, BD Vacutainer tube or equivalent

Preferred Volume

0.5mL

Specimen Minimum Volume

0.2mL

Reject Due To

N/A

Day(s) Performed

24 hours, 7 days a week including holidays.

Method Name

Spectrophotometric, Kinetic (hexokinase)

CPT

82951, 82952 x3

Fee Code

43108, 43375 x3

Test Usage

The GGTT is a diagnostic test for
diabetes in pregnant patients, usually between 24 and 28 weeks of
pregnancy. Venous plasma glucose is measured in a fasting patient
before and at 1, 2, and 3 hour intervals after an oral dose of 100
gm of glucose.

Offsite Collection Instructions

To be followed in order as listed below.
1. Patient Fasting: The patient is to eat no food after
midnight
prior to the test (fasting 8-14 hours before test).
2. Fasting Draw:
a. A fasting blood sample should be drawn into a gray top
Vacutainer tube before Glucocrush® 100 ingestion.
b. Sample is labeled GGTT Fasting or
GGTT-Fast.
3. Glucose Beverage Ingestion:
a. Patient drinks the entire bottle of refrigerator-temperature
Glucocrush? 100 solution from a paper cup within 5 minutes. The
patient may pour the Glucocrush? 100 over ice if so desired.
b. Timing begins the moment the patient starts drinking.
c. Patient is to remain seated as much of the time as possible
and
is not to chew gum, smoke, drink or eat during the test.
Ingestion
of small amounts of water is acceptable.
4. Patient Instructions:
a. A copy of the draw schedule will be provided to the patient
and
she will be instructed to return for blood draws 5 minutes prior
to
the designated specimen collection times.
b. If the patient feels ill, or if she regurgitates, the staff
will
be notified immediately in order to contact the patient’s
physician for further instructions. If the physician instructs
to
complete the test, this will be noted on the requisition
5. Additional Draws/Time Intervals:
The remaining blood samples are also collected in gray top
tubes
and labeled as follows (in minutes after glucose ingestion
began):
• At 1 hour (60 min. after glucose ingestion) label GGTT60
• At 2 hours (120 min. after glucose ingestion) label
GGTT120
• At 3 hours (180 min. after glucose ingestion) label
GGTT180
6. Storage/Transport:
All blood samples are kept at room temperature until the test
is
completed. Then all the samples are sent together, immediately
to
Laboratory Service Center.

Refer to Glucose Tolerance testing protocol in the Glucose
Tolerance Testing Collection link

(make the part in blue a link to this document)

UCMC Collection Instructions

To be followed in order as listed below.
1. Patient Fasting: The patient is to eat no food after
midnight
prior to the test (fasting 8-14 hours before test).
2. Fasting Draw:
a. A fasting blood sample should be drawn into a gray top
Vacutainer tube before Glucocrush® 100 ingestion.
b. Sample is labeled GGTT Fasting or
GGTT-Fast.
3. Glucose Beverage Ingestion:
a. Patient drinks the entire bottle of refrigerator-temperature
Glucocrush? 100 solution from a paper cup within 5 minutes. The
patient may pour the Glucocrush? 100 over ice if so desired.
b. Timing begins the moment the patient starts drinking.
c. Patient is to remain seated as much of the time as possible
and
is not to chew gum, smoke, drink or eat during the test.
Ingestion
of small amounts of water is acceptable.
4. Patient Instructions:
a. A copy of the draw schedule will be provided to the patient
and
she will be instructed to return for blood draws 5 minutes prior
to
the designated specimen collection times.
b. If the patient feels ill, or if she regurgitates, the staff
will
be notified immediately in order to contact the patient’s
physician for further instructions. If the physician instructs
to
complete the test, this will be noted on the requisition
5. Additional Draws/Time Intervals:
The remaining blood samples are also collected in gray top
tubes
and labeled as follows (in minutes after glucose ingestion
began):
• At 1 hour (60 min. after glucose ingestion) label GGTT60
• At 2 hours (120 min. after glucose ingestion) label
GGTT120
• At 3 hours (180 min. after glucose ingestion) label
GGTT180
6. Storage/Transport:
All blood samples are kept at room temperature until the test
is
completed. Then all the samples are sent together, immediately
to
Laboratory Service Center.

Refer to Glucose Tolerance testing protocol in the Glucose
Tolerance Testing Collection link

(make the part in blue a link to this document)

Critical Results

<40 mg/dL
>500mg/dL

Stability: Room Temp

8 hours

Stability: Refrigerated

72 hours

Stability: Frozen

Not Stable

Reference Values

Any two results reaching or exceeding the following limits
for
a
100 gram oral glucose load are generally considered definitive
for
the diagnosis of gestational diabetes mellitus (GDM); however, exceptions
can occur (see references
below):

Fasting level 95 mg/dL
1 hour level of 180 mg/dL
2 hour level of 155 mg/dL
3 hour level of 140 mg/dL

REFERENCES:
1. Gestational diabetes mellitus.Diabetes Care 26 (Suppl 1);
S103-S105, 2010.
2. Diagnosis and Classification of diabetes mellitus. Diabetes
Care
;2033 (Suppl 1):S62-S69, 2010

Pediatric Volume

0.2mL

Clinical Indications

The GGTT is
a diagnostic test for
diabetes in pregnant patients, usually between 24 and 28 weeks
of
pregnancy. Venous plasma glucose is measured in a fasting
patient
before and at 1, 2, and 3 hour intervals after an oral dose of
100
gm of glucose.

Transport Instructions

To be followed in order as listed below.
1. Patient Fasting: The patient is to eat no food after
midnight
prior to the test (fasting 8-14 hours before test).
2. Fasting Draw:
a. A fasting blood sample should be drawn into a gray top
Vacutainer tube before Glucocrush?? 100 ingestion.
b. Sample is labeled GGTT Fasting or
GGTT-Fast.
3. Glucose Beverage Ingestion:
a. Patient drinks the entire bottle of refrigerator-temperature
Glucocrush? 100 solution from a paper cup within 5 minutes. The
patient may pour the Glucocrush? 100 over ice if so desired.
b. Timing begins the moment the patient starts drinking.
c. Patient is to remain seated as much of the time as possible
and
is not to chew gum, smoke, drink or eat during the test.
Ingestion
of small amounts of water is acceptable.
4. Patient Instructions:
a. A copy of the draw schedule will be provided to the patient
and
she will be instructed to return for blood draws 5 minutes
prior
to
the designated specimen collection times.
b. If the patient feels ill, or if she regurgitates, the staff
will
be notified immediately in order to contact the patient�??s
physician for further instructions. If the physician instructs
to
complete the test, this will be noted on the requisition
5. Additional Draws/Time Intervals:
The remaining blood samples are also collected in gray top
tubes
and labeled as follows (in minutes after glucose ingestion
began):
�?� At 1 hour (60 min. after glucose ingestion) label GGTT60
�?� At 2 hours (120 min. after glucose ingestion) label
GGTT120
�?� At 3 hours (180 min. after glucose ingestion) label
GGTT180
6. Storage/Transport:
All blood samples are kept at room temperature until the test
is
completed. Then all the samples are sent together, immediately
to
Laboratory Service Center.

Refer to Glucose Tolerance testing protocol in the Glucose
Tolerance Testing Collection link

(make the part in blue a link to this document)

Test Components

N/A

Specimen Stability

8 hours

Methodology

Spectrophotometric, Kinetic (hexokinase)