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Test Code LS3 Lymphocyte Subset 3

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: not applicable 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimen at room temperature. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 

 

Reference Values

Analyte

Age

Sex

Reference Range

Absolute lymphocyte count

3 months

Male and female

3400 – 7600

Absolute lymphocyte count

6 months

Male and female

3900 – 9000

Absolute lymphocyte count

1 year

Male and female

3400 – 9000

Absolute lymphocyte count

2 years

Male and female

3600 – 8900

Absolute lymphocyte count

6 years

Male and female

2300 – 5400

Absolute lymphocyte count

12 years

Male and female

1900 – 3700

Absolute lymphocyte count

12 years and up

Male and female

1200 – 3265

CD3 percent

3 months

Male and female

53 – 84

CD3 percent

6 months

Male and female

51 – 77

CD3 percent

1 year

Male and female

49 – 76

CD3 percent

2 years

Male and female

53 – 75

CD3 percent

6 years

Male and female

56 – 75

CD3 percent

12 years

Male and female

60 – 76

CD3 percent

12 years and up

Male and female

61 – 82

CD3 absolute count

3 months

Male and female

2500 – 5500

CD3 absolute count

6 months

Male and female

2500 – 5600

CD3 absolute count

1 year

Male and female

1900 – 5900

CD3 absolute count

2 years

Male and female

2100 – 6200

CD3 absolute count

6 years

Male and female

1400 – 3700

CD3 absolute count

12 years

Male and female

1200 – 2600

CD3 absolute count

12 years and up

Male and female

828 – 2328

CD4 percent

3 months

Male and female

35 – 64

CD4 percent

6 months

Male and female

35 – 56

CD4 percent

1 year

Male and female

31 – 56

CD4 percent

2 years

Male and female

32 – 51

CD4 percent

6 years

Male and female

28 – 47

CD4 percent

12 years

Male and female

31 - 47

CD4 percent

12 years and up

Male and female

35 – 60

CD4 absolute count

3 months

Male and female

1600 – 4000

CD4 absolute count

6 months

Male and female

1800 – 4000

CD4 absolute count

1 year

Male and female

1400 – 4300

CD4 absolute count

2 years

Male and female

1300 – 4300

CD4 absolute count

6 years

Male and female

700 – 2220

CD4 absolute count

12 years

Male and female

650 – 1500

CD4 absolute count

12 years and up

Male and female

515 – 1642

CD8 percent

3 months

Male and female

12 – 28

CD8 percent

6 months

Male and female

12 – 23

CD8 percent

1 year

Male and female

12 – 24

CD8 percent

2 years

Male and female

14 – 30

CD8 percent

6 years

Male and female

16 – 30

CD8 percent

12 years

Male and female

18 – 35

CD8 percent

12 years and up

Male and female

14 – 38

CD8 absolute count

3 months

Male and female

560 – 1700

CD8 absolute count

6 months

Male and female

590 – 1600

CD8 absolute count

1 year

Male and female

500 – 1700

CD8 absolute count

2 years

Male and female

620 – 2000

CD8 absolute count

6 years

Male and female

490 – 1300

CD8 absolute count

12 years

Male and female

370 – 1100

CD8 absolute count

12 years and up

Male and female

212 – 887

CD19 percent

3 months

Male and female

6 – 32

CD19 percent

6 months

Male and female

11 – 41

CD19 percent

1 year

Male and female

14 – 37

CD19 percent

2 years

Male and female

16 – 35

CD19 percent

6 years

Male and female

14 – 33

CD19 percent

12 years

Male and female

13 – 27

CD19 percent

12 years and up

Male and female

6 – 22

CD19 absolute count

3 months

Male and female

300 – 2000  

CD19 absolute count

6 months

Male and female

430 – 3000

CD19 absolute count

1 year

Male and female

610 – 2600

CD19 absolute count

2 years

Male and female

720 – 2600

CD19 absolute count

6 years

Male and female

390 – 1400

CD19 absolute count

12 years

Male and female

270 – 860

CD19 absolute count

12 years and up

Male and female

103 – 581

CD56 percent

3 months

Male and female

4 – 18

CD56 percent

6 months

Male and female

3 – 14

CD56 percent

1 year

Male and female

3 – 15

CD56 percent

2 years

Male and female

3 – 15

CD56 percent

6 years

Male and female

4 – 17

CD56 percent

12 years

Male and female

4 – 17

CD56 percent

12 years and up

Male and female

1 – 21

CD56 absolute count

3 months

Male and female

170 – 1100

CD56 absolute count

6 months

Male and female

170 – 830

CD56 absolute count

1 year

Male and female

160 – 950

CD56 absolute count

2 years

Male and female

180 – 920

CD56 absolute count

6 years

Male and female

130 – 720

CD56 absolute count

12 years

Male and female

100 – 480

CD56 absolute count

12 years and up

Male and female

20 – 360

CD4:CD8 Ratio

3 months

Male and female

1.3 – 5.3

CD4:CD8 Ratio

6 months

Male and female

1.5 – 4.7

CD4:CD8 Ratio

1 year

Male and female

1.3 – 4.7

CD4:CD8 Ratio

2 years

Male and female

1.1 – 3.6

CD4:CD8 Ratio

6 years

Male and female

0.9 – 2.9

CD4:CD8 Ratio

12 years

Male and female

0.9 – 4.3

CD4:CD8 Ratio

12 years and up

Male and female

0.9 – 3.3

Critical Values

not applicable

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: not available 

 

Routine Turnaround Time: 24 hours

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm

CPT Codes

86355, 86356 x8, 86360, 86359

LOINC Codes

ABLY 30364-4, CD 33719-6 

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 24 hours 

Refrigerated

(2 - 10°C)

 not acceptable

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

Absolute lymphocyte count, CD3 percent, CD3 absolute count, CD4 percent, CD4 absolute count, CD8 percent, CD8 absolute count, CD4:CD8 ratio, CD19 percent, CD19 absolute count, CD56 percent, CD56 absolute count, CD16 percent, CD16 absolute count, CD3+CD4+CD25+ percent, CD3+CD4+CD25+ absolute count, CD3+CD8+CD25+ percent, CD3+CD8+CD25+ absolute count, CD3+CD4+CD45RA+ percent, CD3+CD4+CD45RA+ absolute count, CD3+CD4+CD45RO+ percent, CD3+CD4+CD45RO+ absolute count, CD1 percent, CD1 absolute, CD5 percent, CD5 absolute count, HLADR percent, and HLADR absolute 

Clinical Indications

  • Assessing the status of the lymphoid immune system in patients with primary immunodeficiencies 
  • Comprehensive Immune Monitoring
  • Immune Reconstitution 

Methodology

Flow cytometry

Synonyms/Search Keywords

Primary immunodeficiency panel, T, B, NK, Native T, Comprehensive Immune Monitoring, Immune Reconstitution 

Last Reviewed Date

8/12/2024