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Test Code LENT Enterovirus, Molecular Detection, PCR, Varies


Advisory Information


This test will detect enterovirus, but will not differentiate viruses in this family or provide serotyping information.

Necessary Information


1. Specimen source is required.

2. Source information should include main anatomical site of collection.



Specimen Required


Submit a raw clinical sample (not a culture isolate) for enterovirus PCR.

 

Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Pericardial, peritoneal

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-M4-RT (T605)

Sources: Dermal, eye, rectal, genital, urethral

Container/Tube: Multimicrobe media (M4-RT) or similar viral transport media (M4 or M5) and Eswab

Specimen Volume: Entire specimen

Collection Instructions:

1. Rectal swab must have no visible fecal matter

2. Place swab back into multimicrobe media (M4-RT, M4, or M5)


Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Testing Algorithm

See Meningitis/Encephalitis Panel Algorithm in Special Instructions.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/RNA Probe Hybridization

Specimen Type

Varies

Specimen Minimum Volume

Body Fluid, Spinal Fluid: 0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Swab Calcium alginate-tipped swab, wood swab, or transport swab containing gel
 

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Sunday; Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87498