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HelpTest Code LABTXHAT (Test code: 857616) HLA Antibody Testing
Ordering Notes
For STAT turn-around time, please send a request to the Transplant Immunology Laboratory by e-mail at HLA-TIILLab@uchicagomedicine.org and/or by pager #8722.
Specimen and Container/Tube
Specimen Type: Serum
Preferred Specimen: Gold top SST (2)
Other Acceptable Specimens: Tiger top (marble) SST, red top
Volume
Preferred Volume: 6mL
Minimum Volume: 6mL
Specimen Stability
Specimens may be kept at room temperature for up to 24 hours following collection. Once centrifuged, specimens may be kept at refrigeraton (4°C) for up to one week.
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Specimen Handling and Transport Instructions
UCM Onsite Instructions: Collect specimen per standard collection procedures and send to the laboratory immediately.
UCM Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedures and send to the laboratory using standard transport procedures.
Rejection Criteria
Unspun samples greater than 24 hours may be rejected. Sample with visible signs of hemolysis (H-Index > 100) may be rejected.
Reference Values
No reference range; results may be provided in an interpretive report.
Critical Values
Not Applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the testing laboratory section.
STAT Turnaround Time: 2 business days
Routine Turnaround Time: 10 business days
If test results are needed same-day, please contact the laboratory at 2-0700, and page #8722.
Testing Schedule: M-F 7:00am-7:00pm
Clinical Indications
In the pre-transplant setting, testing is performed for the detection and characterization of HLA-specific antibodies that could cause potential complications at the time of transplant.
In the post-transplant setting, testing is performed for the routine monitoring of patients for early signs of rejection, which assist with the course of treatment.
Platelet transfusion support: Patients with low platelet counts are at risk for serious bleeding complications and are often transfused with platelets from healthy donors. However, patients can sometimes produce antibodies to the HLA molecules expressed on platelets, which reduce the effectiveness of platelet transfusion. HLA typing, in addition to antibody detection, help physicians choose a platelet donor that will result in increased platelet counts for patients in need.
Test Components
HLA antibody screening: Flow-cytometry using phenoype beads
HLA antibody screening/identification: Solid-phase using phenotype beads
HLA antibody specificity: Solid-phase using single antigen beads
CPT Codes
HLA antibody screening:
HLA Class I and Class II: 86828
HLA Class I or Class II: 86829
HLA antibody screening/identification:
HLA Class I: 86830
HLA Class II: 86831
HLA antibody specificity:
HLA Class I: 86832
HLA Class II: 86833
Adsorption or other serum treatment(s) may apply: 86828-86833/test
Antibody titers:
HLA Class I: 86834/dilution
HLA Class II: 86835/dilution
Methodology
Solid-phase and/or flow cytometry
Synonyms/Key Search Words
HAT, HLA Antibody, HLA, Antibody