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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code IPPNH Paroxysmal Nocturnal Hemoglobinuria (PNH) Evaluation by Flow Cytometry

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: not applicable 

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 24 hours 

Refrigerated

(2 - 10°C)

 5 days

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimens in a refrigerator. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 

 

Reference Values

Analyte

Age

Sex

Reference Range

not applicable

 

 

 

Critical Values

not applicable 

Clinical Indications

  • Testing for Paroxysmal Nocturnal Hemoglobinuria (PNH) diagnosis and follow-up. 

Test Components

CD14 monocytes and CD24 granulocytes, CD59 erythrocytes, pathologist's interpretation 

Methodology

Flow Cytometry 

CPT Codes

88184, 88185 x5, 88187

LOINC Codes

Not applicable 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: not available

 

Routine Turnaround Time: 48 hours

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm

Synonyms/Search Keywords

PNH testing 

Last Reviewed Date

9/5/2024