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The University of Chicago Medicine Laboratories

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Specimen Collection and Handling

Transfusion

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Mayo Clinic Laboratories

Test Code IPPNH Paroxysmal Nocturnal Hemoglobinuria (PNH) Evaluation by Flow Cytometry

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: not applicable 

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

 24 hours 

Refrigerated

(2 - 10°C)

 5 days

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimens in a refrigerator. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 

 

Reference Values

Analyte

Age

Sex

Reference Range

not applicable

 

 

 

Critical Values

not applicable 

Clinical Indications

  • Testing for Paroxysmal Nocturnal Hemoglobinuria (PNH) diagnosis and follow-up. 

Test Components

CD14 monocytes and CD24 granulocytes, CD59 erythrocytes, pathologist's interpretation 

Methodology

Flow Cytometry 

CPT Codes

88184, 88185 x5, 88187

LOINC Codes

Not applicable 

Volume

Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

 

STAT Turnaround Time: not available

 

Routine Turnaround Time: 48 hours

 

Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm

Synonyms/Search Keywords

PNH testing 

Last Reviewed Date

9/5/2024