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HelpTest Code IPPNH Paroxysmal Nocturnal Hemoglobinuria (PNH) Evaluation by Flow Cytometry
Specimen and Container/Tube
Specimen Type: Whole blood
Preferred Specimen: lavender top
Other Acceptable specimens: not applicable
Specimen Handling and Transport Instructions
UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.
UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimens in a refrigerator.
Rejection Criteria
- Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
- Frozen or heated specimens will be rejected
- Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected
Reference Values
|
Analyte |
Age |
Sex |
Reference Range |
|
not applicable |
|
|
|
Critical Values
not applicable
Turnaround Time
Turnaround times are relative to the time the specimen is received in the test laboratory.
STAT Turnaround Time: not available
Routine Turnaround Time: 48 hours
Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm
CPT Codes
88184, 88185 x5, 88187
LOINC Codes
Not applicable
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 1.0 mL
Specimen Stability
|
Storage Temperature |
Stability |
|
Room temperature (20 - 25°C) |
24 hours |
|
Refrigerated (2 - 10°C) |
5 days |
|
Frozen (-20°C) |
not acceptable |
Collection Instructions
Collect specimen per standard laboratory collection procedures.
Test Components
CD14 monocytes and CD24 granulocytes, CD59 erythrocytes, pathologist's interpretation
Clinical Indications
- Testing for Paroxysmal Nocturnal Hemoglobinuria (PNH) diagnosis and follow-up.
Methodology
Flow Cytometry
Synonyms/Search Keywords
PNH testing
Last Reviewed Date
9/5/2024