Test Code HDL High Density Lipoprotein Cholesterol

Specimen and Container/Tube

Specimen Type: Plasma

Preferred Specimen: Li-Heparin Mint Green Top

Other Acceptable specimens: Serum/Gold Top, Serum/Red Top

Specimen Handling and Transport Instructions

UCMC Onsite Instructions:

Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions:

Samples should be centrifuged within 2 hours of collection.
Centrifuge specimens at >2500 x g for 10 minutes at room temperature.
Aliquot sample into plastic transport tube containing a minimum of 0.2 mL of specimen.
Samples must be sent room temperature at 15-25^oC.

Rejection Criteria

Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits.
Specimens that are clotted or grossly hemolyzed.
Specimens that were transported unspun/whole blood and draw time is greater than 6 hours.

Reference Values

Test	Age	Sex	Reference Range
HDL Cholesterol	All	All	40 - 80 mg/dL

Note: Reference range based on in-house studies, recommendations from Tietz Textbook of Clinical Chemistry, and Roche product literature. Reference ranges for cholestrol are based on a general adult patient population. Cholesterol levels vary with age, sex and race.

Critical Values

Analyte	Unit	Low	High	Age
N/A	N/A	N/A	N/A	N/A

CPT Codes

83718

LOINC Codes

2085-9

Volume

Preferred Volume: 0.5 mL

Minimum Volume: 0.2 mL

Specimen Stability

Storage Temperature	Stability
Room temperature	72 hours
Refrigerated	7 days
Frozen (-15 to -25°C) Frozen (-70°C)	3 months 24 months

Collection Instructions

Collect specimen per standard laboratory collection procedures.

Test Components

HDL Cholesterol

Clinical Indications

High-density lipoprotein cholesterol (HDL-C) is associated with lower risk of cardiovascular disease. Excess cholesterol is actively pumped into HDL to be carried in the blood circulation and cleared by the liver in a process known as reverse cholesterol transport. For these reasons, HDL-C is often referred to as "good" cholesterol.

Methodology

Enzymatic Colorimetric (Roche Diagnostics)

Additional Information

HDL-C is rarely measured in isolation and most often ordered along with total cholesterol and triglycerides. Measuring HDL-C and total cholesterol enables calculation of non-HDL cholesterol (total cholesterol-HDL-C). Non-HDL cholesterol is the combination of low-density lipoprotein cholesterol and very-low density lipoprotein cholesterol. Non-HDL cholesterol is directly associated with risk for cardiovascular disease and referred to as "bad" cholesterol.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory.

STAT Turnaround Time: Not available as STAT test

Routine Turnaround Time: 4 hours

Testing Schedule: 24/7

Synonyms/Keyword Search

Cholesterol, HDL (High Density Lipoprotein)

HDL Direct

High Density Lipoprotein, Cholesterol

HDL (High Density Lipoprotein), Cholesterol