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Test Code HBEV Hemoglobinopathy Evaluation

Specimen Type



Lavender top

Preferred Volume

4 mL


Monday – Friday 7:00 a.m. – 3:00 p.m.

Turnaround Time

24 to 72 hours; 7 – 10 days for uncommon variants

STAT Availability

Not Available

Test Usage

Screening and quantitation of hemoglobins A, A2, F and hemoglobin variants by HPLC.

Test Methodology

High performance liquid chromatography (HPLC)

Additional Information

Elevated levels of hemoglobin A2 may be masked by concurrent iron deficiency anemia. If beta-thalassemia is suspected, but the hemoglobin A2concentration is normal, the patient should be evaluated for iron deficiency. If iron deficiency is found and if microcytosis persists after adequate iron therapy, the hemoglobin A2 concentration should be rechecked. In hemoglobin Lepore trait and in alpha-thalassemia, the proportion of hemoglobin A2may be diminished. Patients with megaloblastic anemias may have increased concentrations of hemoglobin A2 


Fetal hemoglobin values may be elevated in sickle cell disease and beta thalassemia conditions as well as in patients with hereditary persistence of fetal hemoglobin.

CPT Code


Test Includes

Screening and quantitation of hemoglobins A, A2, F and hemoglobin variants.

Critical Results


Test Limitations

For confirmation and specific identification of uncommon hemoglobin variants (non-S/non-C), an additional specimen must be submitted to Laboratory Referral Services for sendout to reference laboratory.

Reference Range

No variants detected at any age.

Results are subject to ordering physician’s interpretation for identification of common  hemoglobinopathies and thalassemias.


Age HbA HbA2 HbF
Newborn to 1 month 4.0-50.0% <1.0% 50.0-95.0%
1 to 2 months 11.5-74.5% 0.5-3.5% 25.0-85.0%
2 to 3 months 36.5-89.5% 0.5-3.5% 10.0-60.0%
3 to 4 months 61.5-94.5% 0.5-3.5% 5.0-35.0%
4 to 6 months 76.5-99.5% 0.5-3.5% <20.0%
6 to 12 months 86.5-99.5% 0.5-3.5% <10.0%
> 12 months 94.5-98.0% 2.0-3.5% <2.0%

Minimum Volume

2 mL

Fee Code



Hemoglobin Screen; Hemoglobin Electrophoresis