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Test Code CTBID Culture Referred for Identification, Mycobacterium and Nocardia, Varies

Infectious

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Specimen Type: Mycobacterium species or aerobic actinomycetes organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume:

Solid media: Visible growth of isolate

Isolate in broth media: ≥3 mL

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.

Additional Information: A minimum volume of 3 mL is recommended to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is less than 3 mL, initial testing may be limited, and increased turnaround time is likely.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Testing Algorithm

Reflex tests may be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.

 

See Culture Referred for Identification Mycobacterium and Nocardia Algorithm.

Reject Due To

Agar plate Reject
Isolates other than Mycobacterium species or aerobic actinomycetes Reject

Report Available

60 to 70 days

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RMALM Id MALDI-TOF Mass Spec AFB No, (Bill Only) No
RTBSP Id, Mtb Speciation, PCR No, (Bill Only) No
ISMY ID by 16S Sequencing No, (Bill Only) No
LCTB Id, MTB complex Rapid PCR No, (Bill Only) No

CPT Code Information

Culture Referred for Identification, Mycobacterium

87118-Identification of mycobacteria

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

Method Name

Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)/16S rDNA Sequencing/Rapid Polymerase Chain Reaction (PCR)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID

80278

Specimen Minimum Volume

See Specimen Required