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Test Code COVID19 SARS-CoV-2 (COVID-19) Qualitative PCR

Important Note

Order this test using the AgileMD Clinical Pathway.

Additional Codes

COVID-19

COVID19

Novel Coronavirus

Specimen and Container/Tube

Acceptable Specimen Types

Flocked nasopharyngeal swab in Universal Viral Transport or Flocked nasal swab in liquid Amies transport media.

No other specimens types can be tested.

Specimen Handling and Transport Instructions

See UCMC collection instructions

Transport refrigerated if >2 hrs from time of collection.

Reference Values

Negative

Critical Values

 

All positive  culture

 

 

 

 

All

Turnaround Time

Day(s) Performed

Monday through Sunday

LOINC Codes

30687635

Collection Instructions

UCMC Collection Instructions

Specimen SourceFlocked nasopharyngeal swab; 1 specimen/day

Specimen Collection: Kit consists of flocked nasopharyngeal swab and viral transport media.

  1. Tilt the patient’s head back 70 degrees.
  2. Insert the flocked swab through the nostril parallel to the palate (not upwards) until resistance is encountered and the swab reaches a depth equal to the distance from the nostrils to the outer opening of the ear.
  3. Rotate the flocked swab 2-3 times and hold the swab in place for 5-10 seconds to ensure maximum absorbency.   Specimens can be collected from both sides using the same swab, but it is not necessary to collect specimens from both sides if the minitip is saturated with fluid from the first collection.
  4. Put the swab in the viral transport media and break the shaft at the designated breakpoint.
  5. Tightly close the lid.


Specimen TransportAmbient temperature if < 2 hr, transport refrigerated up to 48 hours from time of collection. If more than 48 hours, freeze at -70°C or colder.

Test Components

Flocked nasopharyngeal swab in Universal Viral Transport or Flocked nasal swab in liquid Amies transport media.

No other specimens types can be tested.

Methodology

This test is performed using the Roche cobas SARS-CoV-2 assay on the cobas 6800,  the Cepheid SARS-CoV-2 assay on the GeneXpert or the BioFire SARS-CoV-2 assay on the Torch. These assays are for use only under Emergency Use Authorization (EUA) in U.S. laboratories certified under the Clinical Laboratories Improvement Amendments of 1988 (CLIA). The University of Chicago Clinical Microbiology and Immunology Laboratory is certified under CLIA to perform high complexity tests.

Synonyms/Search Keywords

COVID-19

COVID19

Novel Coronavirus

Last Reviewed Date

08/16/2022

Additional Information

Order this test using the AgileMD Clinical Pathway.