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Test Code BETA2 Beta-2 Transferrin: Detection of Spinal Fluid in Other Body Fluid

Important Note

Use Sunquest code BTRANS to order

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Body Fluid


Additional Testing Requirements


If specimens are collected from multiple sites on the body (ie, left and right), each specimen must be sent under a separate order.



Necessary Information


Indicate specimen source. Include whether the source is from the right or left side of the body, if applicable.



Specimen Required


Specimen Type: Body fluid

Sources: Nasal, otic, wound, etc

Container/Tube:

Preferred: Sterile container, syringe, test tube, or microtube

Acceptable: Plain cotton swab or gauze

Specimen Volume: 0.5 mL

Collection Instructions:

1. If submitting a syringe, remove needle. Add cap to end of syringe.

2. If direct collection is not feasible, specimen may be collected using a plain cotton swab or gauze.

3. If gauze is used to collect specimen, circle area on the gauze where specimen was collected.

4. Place cotton swab or gauze in as small a container as possible (eg, plain test tube or collection container).

5. Do not collect specimen with a culture swab.

6. Do not add any liquid to the swab or gauze.

Additional Information:

1. Specimens collected from above the shoulders risk salivary contamination, which can degrade the beta-2 transferrin protein. These specimens should be frozen immediately following collection and kept frozen until testing is performed.

2. Although results may be obtainable on smaller specimens (perhaps as little as 0.05 mL, depending on the protein concentrations and percentage of spinal fluid in the specimen), reliable results are best obtained with an adequate specimen volume.

3. Specimens collected with additives such as microbiology media (eg, Stuart or Amies liquid medium) or TransFix/EDTA (used for analyses in flow cytometry) yield uninterpretable results and will be rejected.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Body Fluid Frozen (preferred)
  Ambient 
  Refrigerated 

Reference Values

Negative, no beta-2 transferrin (spinal fluid) detected

Day(s) Performed

Monday through Friday

CPT Code Information

86335

Report Available

1 to 3 days

Method Name

Electrophoresis/Immunofixation

Secondary ID

80351