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HelpTest Code ANST Androstenedione, Serum
Testing Algorithm
For more information see Steroid Pathways.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum Red8 am draw is recommended
After patient has been active for 2 hours
Preferably no later than 10am.
Ordering Guidance
Additional Testing Requirements
For diagnosis and differential diagnosis of hyperandrogenism, an initial workup in adults should also include total and bioavailable testosterone (TTBS / Testosterone, Total and Bioavailable, Serum) measurements. Depending on results, this may be supplemented with measurements of sex hormone-binding globulin (SHBG1 / Sex Hormone-Binding Globulin, Serum) and other androgenic steroids (eg, dehydroepiandrosterone sulfate [DHEA-S]).
For diagnosis of congenital adrenal hyperplasia (CAH), the following assays should also be ordered:
-OHPG / 17-Hydroxyprogesterone, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-CORT / Cortisol, Serum
For monitoring CAH treatment, the following assays should also be ordered:
-TTST / Testosterone, Total, Mass Spectrometry, Serum
-OHPG / 17-Hydroxyprogesterone, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-DHEA_ / Dehydroepiandrosterone [DHEA], Serum.
For diagnosis of premature adrenarche, the following assays should also be ordered:
-FSH / Follicle-Stimulating Hormone [FSH], Serum
-LH / Luteinizing Hormone [LH], Serum
-TTBS / Testosterone, Total and Bioavailable, Serum or TGRP / Testosterone, Total and Free, Serum
-EEST / Estradiol, Serum
-DHES1 / Dehydroepiandrosterone Sulfate, Serum
-DHEA_ / Dehydroepiandrosterone (DHEA), Serum
-SHBG1 / Sex Hormone-Binding Globulin, Serum
-OHPG / 17-Hydroxyprogesterone, Serum
Specimen Required
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 7 days |
Offsite Collection Instructions:
8 am draw is recommended
After patient has been active for 2 hours
Preferably no later than 10am.
Special Instructions
Reference Values
PEDIATRICS*
Premature infants
26-28 weeks, day 4: 92-282 ng/dL
31-35 weeks, day 4: 80-446 ng/dL
Full-term infants
1-7 days: 20-290 ng/dL
1 month-1 year: <69 ng/dL
Males*
|
Tanner stages |
Age (Years) |
Reference range (ng/dL) |
|
Stage I (prepubertal) |
<9.8 |
<51 |
|
Stage II |
9.8-14.5 |
31-65 |
|
Stage III |
10.7-15.4 |
50-100 |
|
Stage IV |
11.8-16.2 |
48-140 |
|
Stage V |
12.8-17.3 |
65-210 |
Females*
|
Tanner stages |
Age (Years) |
Reference range (ng/dL) |
|
Stage I (prepubertal) |
<9.2 |
<51 |
|
Stage II |
9.2-13.7 |
42-100 |
|
Stage III |
10.0-14.4 |
80-190 |
|
Stage IV |
10.7-15.6 |
77-225 |
|
Stage V |
11.8-18.6 |
80-240 |
*Soldin SJ, Brugnara C, Wong EC, eds. Androstenedione. In: Pediatric Reference Ranges. 4th ed. AACC Press; 2003: 32-34
ADULTS
Males: 40-150 ng/dL
Females: 30-200 ng/dL
For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
Day(s) Performed
Monday through Friday
CPT Code Information
82157
Report Available
2 to 5 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)