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Test Code AFBB Culture, Blood, Acid Fast Bacilli (AFB)

Important Note

 

AFB culture only. No stain.

Specimen and Container/Tube

 

Specimen Type: Whole blood

Preferred Specimen: Blood Culture Bottles

Other Acceptable specimens: not applicable 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 2 hours. If transport is delayed, refrigerate specimens prior to transport. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or refrigerated specimens will be rejected
  • Specimen in incorrect specimen collection container will be rejected.

Reference Values

Analyte

Age

Sex

Reference Range

Culture

All

Male and female

Negative

Critical Values

All positive culture

If positive, Microbiology lab will notify physician.

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 

STAT Turnaround Time: not avialable

 

Routine Turnaround Time: 5 - 6 days

 

Testing Schedule: 24/7

 

CPT Codes

87102

LOINC Codes

30687102

Volume

Preferred Volume:  8-10mL

Minimum Volume: 1-3 mL

Specimen Stability

Storage Temperature

Stability

Room temperature

(20 - 25°C)

24 hours 

Refrigerated

(2 - 10°C)

not acceptable

Frozen

(-20°C)

not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. Skin decontamination with Chloraprep.

Pinch wings of the applicator to break the ampule inside. Wet sponge by repeatedly pressing and releasing sponge against venipuncture site. Scrub venipuncture site continuously for 30 seconds using a back and forth motion. Allow the site to air dry for 30 seconds. DO NOT blot or dry. To prepare the bottle, remove the plastic cap from the top of the bottles and cleanse with 70% alcohol. Remove any surplus disinfectant with sterile gauze before injection of blood into the bottle.

Specimen Transport: Immediate, no refrigeration.

Clinical Indications

Infection of Mycobacteria species

 

Methodology

Automated culture method

Bactec FX40 Automated culture method

Synonyms/Search Keywords

Mycobacteria Culture

Blood culture for Acid Fast Bacilli

Last Reviewed Date

08/15/2022