Test Code ACST Acanthamoeba Stain
Specimen and Container/Tube
Corneal scraping, Eye, Contact lens solution and case
Glass slides obtained from the Microbiology lab (2-6133)
Volume
Preferred Volume: 4.0 mL
Minimum Volume: 1.0 mL
Specimen Stability
Specimens other than blood and tissue should be refrigerated
Collections Instructions
Offsite Collection Instructions
See UCMC Collection Instructions
UCMC Collection Instructions
Specimen Source: Corneal scraping
Specimen Container: Glass slides obtained from the Microbiology
lab. Please call the lab at 773-702-6133.
Specimen Collection: Specimen collected by ophthalmologist using a
sterile spatula to scrape ulcers and/or lesions. Scrapings are
directly inoculated onto glass media (between the frosted ring).
Please label the frosted part of the slide with patient
information.
Specimen Transport: 24 hr, no refrigeration
Specimen Source: Contact lenses, solution, and
case
Specimen Collection: Send contact lenses, contact cases, and/or
contact solution to Microbiology lab.
Specimen Transport: 24 hr, no refrigeration
See MicrobiologySpecimen
Collection:
- Category “Eye” in Microbiology Specimen
CollectionTables, Table 1 “Bacteriology, Chlamydia,
Mycobacteriology (AFB), Mycology, Virology”
Specimen Handling and Transport Instructions
Transport Instructions
See UCMC Collection Instructions
Rejection Criteria
Whole blood/unspun specimens greater than 6 hours will be
rejected.
Reference Values
Negative
Critical Values
All positive culture
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Turnaround Time
Day(s) Performed
Monday through Saturday
STAT DAY(S) AND TIME(S) PERFORMED
Test Components
Corneal scraping, Eye, Contact lens solution and case
Methodology
Fluorescent stain (Acridine Orange Stain)
CPT Codes
87206
LOINC Codes
30187206
Last Review Date
09/6/2023