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Test Code TE Testosterone, Total

Specimen Type



Red top

Preferred Volume

4 mL


Tuesday and Friday

Turnaround Time

4 – 5 days

STAT Availability

Not Available

Test Methodology

high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS)

Additional Information

This test was developed and its performance characteristics determined by the University of Chicago Medical Center, Clinical Chemistry Laboratories in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code


STAT Turnaround Time

Not available

Reference Range

Age-Adjusted Ranges Reference Range (ng/dL)



0-5 months 75-400

6 months-9 years <20

10-11 years <130

12-13 years <800

14 years <1,200

15-16 years 100-1,200

17-18 years 300-1,200

19-55 years 240-950§

56-75 years 180-800§



0-5 months 20-80

6 months-9 years <20

10-11 years <44

12-16 years 15-60†

17-40 years 20-60†

41-60 <60


Post-menopausal (>60 yrs) 5-70§


†Female total testosterone reference ranges established in children and women by studies performed at UCMC1,2,3.

§Testosterone data in the elderly are informational, based on radioimmunoassay data.




1. Children: Bordini BD, Littlejohn EE, Rosenfeld RL. J Clin Endocrinol Metab 2009; 94: 1168-1175
2. Women: Mortensen M, Ehrmann DA, Littlejohn E, Rosenfield RL. J Clin Endocrinol Metab 2009; 94: 1579-86
3. Rosenfield RL, Mortensen M, Wroblewski K, Littlejohn E, Ehrmann DA. Determination of the source of androgen excess in functionally atypical polycystic ovary syndrome by a short dexamethasone androgen-suppression test and a low-dose ACTH test. Hum Reprod 2011;26:3138-46

Critical Results


Test Limitations

Testosterone levels can fluctuate substantially between different days, and sometimes even more frequently. Assessment of androgen status should be based on more than a single measurement.

Minimum Volume

2 mL

Test Includes

quantitative determination of Total testosterone

Fee Code