Test Code LAAPS2 LA/APS Level 2 Antigenic Package

Specimen Type

Blood

Container

1 Red top tube

Preferred Volume

6 mL

Offsite Collection Instructions

Whole blood or serum samples might be sent to the laboratory.

Centrifugation instructions:

Serum specimen should be centrifuged at >1500 x g for 10 minutes.
Aliquot removed serum into 2 tubes containing minimum of 0.5 mL of specimen each. They may be sent refrigerated or frozen.

Note: Frozen samples that have thawed in transit will be rejected.

UCMC Collection Instructions

Specimens for level 2 antigenic testing can be collected and sent to the lab at any time.

Availability

Testing will be performed on Wednesday of each week. Samples to be tested should be in the lab no later than 8:30 am on Wednesday in order to be included in that run

Turnaround Time

7 days

STAT Availability

Not Available

Test Usage

The package includes LA antigenic testing for detection of “antiphospholipid” antibodies.

Test Methodology

ELISA

CPT Code

86147, 86146, 86148

Test Includes

The package includes antigenic testing for IgA antibodies directed against beta-2 GPI and against cardiolipin. It additionally includes antigenic testing for IgG and IgM antibodies directed againstphosphatidylserine/prothrombin complexes.

Reference Range

Anticardiolipin IgA: Negative <12.0 APL; Borderline positive 12.0-19 APL; Medium positive 20-80 APL; High positive >80 APL

Beta-2 GPI IgA: <20.1 U/mL

APS/PT IgG: <30.1 U/mL

APS/PT IgM: <30.1 U/mL

Minimum Volume