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Test Code KPNRP KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR

Biohazard Infectious

Reporting Name

KPC and NDM PCR

Useful For

Assessing pure isolates of gram-negative bacilli for mechanism of carbapenem resistance

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.



Necessary Information


Organism identification and specimen source are required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Klebsiella pneumoniae (KPC) or New Dehli metallo-beta-lactamase (NDM) DNA is unlikely.

 

Supplies: Infectious Container, Large (T146)

Collection Container/Tube: Slant

Specimen Volume: Isolate

Collection Instructions:

1. Isolate the bacteria.

2. Bacterial organism must be in pure culture, actively growing. Do not submit mixed cultures.

3. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KPNRP KPC and NDM PCR 85502-3

 

Result ID Test Result Name Result LOINC Value
SRC53 Specimen source 31208-2
35168 KPC PCR 85499-2
35169 NDM PCR 85500-7

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Agar Plate, mixed cultures

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler with Amplified Product Detection Using Fluorescent Resonance Energy Transfer (FRET) Hybridization Probes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).