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Test Code IPPNH NEW Paroxysmal Nocturnal Hemoglobinuria (PNH) Evaluation by Flow Cytometry

Specimen and Container/Tube

Specimen Type: Whole blood

Preferred Specimen: lavender top

Other Acceptable specimens: not applicable 

Specimen Handling and Transport Instructions

UCMC Onsite Instructions: Collect specimen per standard collection procedure and send to laboratory immediately.

UCMC Offsite and UC MedLab Outreach Instructions: Collect specimen per standard collection procedure and send to laboratory immediately. Specimens must be received within 24 hours. If transport is delayed, retain specimens at room temperature. 

Rejection Criteria

  • Common specimen rejected situations include: incorrect specimen type, insufficient volume, missing or incomplete specimen identifiers, incorrect specimen transport, or specimens outside stability limits. 
  • Frozen or heated specimens will be rejected
  • Clotted or quantity not sufficient specimens are not suitable for testing and will be rejected 


Reference Values




Reference Range

not applicable




Critical Values

not applicable 

Turnaround Time

Turnaround times are relative to the time the specimen is received in the test laboratory. 


STAT Turnaround Time: not available


Routine Turnaround Time: 48 hours


Testing Schedule: Monday - Friday 8:00 am - 5:00 pm and Saturday 8:00 am - 3:00 pm

CPT Codes

88184, 88185 x5, 88187


Not applicable 


Preferred Volume: 4.0 mL

Minimum Volume: 1.0 mL

Specimen Stability

Storage Temperature


Room temperature

(20 - 25°C)

24 hours 


(2 - 10°C)

not acceptable



not acceptable

Collection Instructions

Collect specimen per standard laboratory collection procedures. 

Test Components

CD14 monocytes, CD16 and CD24 granulocytes, CD59 erythrocytes, pathologist's interpretation 

Clinical Indications

  • Testing for Paroxysmal Nocturnal Hemoglobinuria (PNH) diagnosis and follow-up. 


Flow Cytometry 

Synonyms/Search Keywords

PNH testing 

Last Reviewed Date