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Test Code HBSAG Hepatitis B Surface Antigen

Important Note

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

Biohazard Infectious

Specimen Type

Serum

Container/Tube

Red Top

Offsite Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be spun and
aliquoted
3. Transport refrigerated (2 – 8˚C)).

Turnaround Time

4 hours

Test Includes

N/A

Preferred Volume

0.5 mL

UCMC Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be received in
Laboratory Service Center
3. Transport room temperature (15 – 25˚C)

STAT Turnaround Time

N/A

Test Usage

The assay may be used in conjunction with other serological and
clinical information to diagnose individuals with acute or chronic
hepatitis B infection.

The assay may also be used to screen for hepatitis B infection
in pregnant women to identify neonates who are at risk of acquiring
hepatitis B during the perinatal period.

Critical Results

N/A

Test Limitations

It has been reported that certain assays will not detect all HBV
mutants. If acute or chronic HBV infection is suspected and the
HBsAg result is nonreactive it is recommended that other HBV
serological markers be tested to confirm the HBsAg
nonreactivity.

The performance of the assay has not been established for
populations of immunocompromised or immunosuppressed patients.

Additional Information

NOTE: Reactive specimens subject to confirmatory testing by
Hepatitis B Surface Antigen Neutralization assay. High level
reactivity does not require confirmation by supplemental Hepatitis
B Surface Antigen Neutralization assay.

Fee Code

30086708

Stability: Room Temp

2 hours

Stability: Refrigerated

5 days

Stability: Frozen

3 months

Included in Additional codes

Also included in AHPNL

Specimen Minimum Volume

0.2 mL

DAY(S) AND TIME(S) PERFORMED

24 hours, 7 days a week including holidays

STAT DAY(S) AND TIME(S) PERFORMED

N/A

CPT

86708

Method Name

Electrochemiluminescence   Immunoassay (ECLIA)

Reference Values

Non-reactive

A nonreactive test result does not exclude the possibility
of
exposure to or infection with hepatitis B virus. A nonreactive
test
result in individuals with prior exposure to hepatitis B may be
due
to antigen levels below the detection limit of this assay or
lack
of antigen reactivity to the antibodies in this assay.

Reject Due To

Whole blood/unspun samples greater than 6 hours will be
rejected.

LI-Heparin tubes will be rejected.

Synonyms

N/A

Coverage Information

N/A