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Test Code GPT Alanine Aminotransferase, Plasma

Patient Preparation

Only use suitable tube or collection containers

Specimen Type

 

Specimen Type

Preferred

Plasma

Acceptable

Serum

 

Specimen Container

 

Specimen Container

Specimen Type

Container

Additive

Plasma

Mint Green

Li Heparin gel separator

Dark Green

Li Heparin

Serum

Red Top

None

Gold Top

Gel  separator

 

Specimen Volume

 

Specimen Volume

Optimum

1.0 mL

Minimum

0.5 mL

 

Specimen Stability

 

Specimen Stability

Specimen Type

Storage Temperature

Stability

Plasma

Room Temperature  (15 - 25˚C)

3 Days

Refrigerated  (2-8˚C)

7 Days

Frozen  (-60 to -80˚C)

>7 Days

Serum

Room Temperature  (15 - 25˚C) 

3 Days 

Refrigerated  (2-8˚C) 

7 Days 

Frozen  (-60 to -80˚C)

>7 Days 

 

Specimen Collection

1. Collect blood form a stasis-free vein using standard collection container.

2. Fill collection tube completely and keep tube tightly stoppered at all times.

3. Separate serum or plasma from the cells or clot promptly.

UCMC Handling and Transport Instructions

1. Unspun whole blood samples need to be received in the Lab Service Center within 2 hours of collection at room temperature.

2. Unspun whole blood samples received in the Lab Service Center after 2 hours of collection must be refrigerated at 2-8°C.

3. If the specimen cannot be sent to the Lab Service Center within 6 hours of collection it must be spun, aliquoted and submitted based on stability requirements (please see the stability section for further information).

Offsite Handling and Transport Instructions

1. Unspun whole blood samples need to be received in the Lab Service Center within 2 hours of collection at room temperature.

2. Unspun whole blood samples received in the Lab Service Center after 2 hours of collection must be refrigerated at 2-8°C.

3. If the specimen cannot be sent to the Lab Service Center within 6 hours of collection it must be spun, aliquoted and submitted based on stability requirements (please see the stability section for further information).

Rejection Criteria

1. Whole blood/unspun specimens greater than 6 hours will be rejected.  

2. If specimens Handling and Transport Criteria are not met, the specimens will be rejected.

3. Specimens will be rejected if stability requirements are not met.  

4. Improper labeling will lead to rejected samples.

Availabiltiy

24 hours, 7 days a week including holidays.

Turnaround Time

Routine Testing: 4 hours

Stat Testing: 1 hour

Test Usage

Assist in the diagnosis of hepatic diseases.

Test Methodology

Spectrophotometric, kinetic

Additional Information

Elevated serum ALT is found in hepatitis, cirrhosis, obstructive jaundice, carcinoma of the liver and chronic alcohol abuse.

Reference Range

8-35 IU/L

Critical Values

Nonce

Test Limitations

Patients with vitamin B6 deficiency may have decreased ALT activity levels. Isoniazid can cause artificially low levels, and Furosemide can cause artificially high levels. Cyanokit (Hydroxycobalamin) and Sulfasalzine may cause interference with results.

CPT Code

84460

Fee Code

30184460

Synonyms

ALT, GPT, SGPT