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Test Code FVLAPC Factor V Leiden/Activated Protein C Resistance

Specimen Type

Blood

Container

1 lavender and 1 light blue top, 3.2% sodium citrate.

Preferred Volume

8 mL

Offsite Collection Instructions

Blue top tube specimen instruction:

 

Whole blood sample must arrive in the laboratory within three hours of specimen collection.

  • If transport time exceeds three hours, follow centrifugation instructions:

    • Samples must be centrifuged at >1500 x g for 10 minutes at room temperature.
    • Carefully remove the plasma from the cells using a plastic transfer pipette avoiding the buffy coat.
    • Aliquot removed plasma into 2 plastic tubes containing a minimum of 500 uL of specimen in each tube.
    • The plasma samples must then be frozen at -20ºC.
    • Once solidly frozen, they must be shipped on dry ice. Samples that have thawed in transit will be rejected.
    Note: For clients who do not have access to dry ice DO NOT freeze the specimen. Follow the centrifugation instructions and send fresh plasma or send whole blood sample. It now becomes vital that the specimen arrives in the lab within three hours of collection.

Do not centrifuge or freeze lavender top tube. Send whole blood specimen.

UCMC Collection Instructions

Sample may be collected at anytime, however testing is performed twice per week.

Availability

Specimens may be collected and sent to the laboratory at any time. Testing is performed twice a week (Tuesday and Thursday) on 1st shift 7:30 am to 3:30 pm.

Turnaround Time

4-5 days

STAT Availability

Not Available

Test Methodology

FVL = Molecular; APCR = Clotting

Additional Information

All 3.2% sodium citrate light blue top tubes require a ratio of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL tube requires 2.7 mL blood added to 0.3 mL of anticoagulant).
 

Initial testing will consist of a functional assay for Activated Protein C (APC) resistance. If an abnormality in the APC resistance testing is found, reflexive testing for the Factor V Leiden gene mutation will also be performed. 

 

Notes:

1. Unlike tests for levels of Protein C, Protein S, or antithrombin, testing for APC resistance is expected to retain validity even in the setting of recent thrombotic events.

2. Absence of an abnormality in the Factor V Leiden gene mutation testing, despite the presence of APC resistance, may be seen in the setting of acquired APC resistance, or in the rare instance of a mutation in Factor V at a locus other than that of the Leiden mutation. Sample can be collected at anytime however testing is batched and performed once or twice per week
 

Volume is 5 mL lavender top tube and 2.7 mL light blue top tube.

CPT Code

APCR: 85307

FVI: 83908

Test Includes

Initial testing will consist of a functional assay for Activated Protein C (APC) resistance. If an abnormality in the APC resistance testing is found, reflexive testing for the Factor V Leiden gene mutation will also be performed.

Reference Range

>2.30; range effective 03/24/17

Minimum Volume

8 mL

Fee Code

APCR: 30085307

FVI: 80038

Synonyms

FVL;APCR; Activated Protein C Resistance