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Test Code F9HM Factor (9) IX for Hemophilia Monitoring

Specimen Type

Plasma

Container/Tube

Light blue top tube, 3.2% sodium citrate

Offsite Collection Instructions

Whole blood sample must arrive in the laboratory within
three hours of specimen collection.

 

If transport time exceeds three hours, follow centrifugation
instructions:

  • Samples must be centrifuged at >2500 x g for 10 minutes
    at room temperature.
  • Carefully remove the plasma from the cells using a plastic
    transfer pipette avoiding the buffy coat.
  • Aliquot removed plasma into 4 plastic tubes containing a
    minimum of 500 uL of specimen in each tube.
  • The plasma samples must then be frozen at -20 C.
  • Once solidly frozen, they must be shipped on dry ice.
    Samples that have thawed in transit will be rejected.

Note: For clients who do not have access to dry ice DO
NOT freeze the specimen. Follow the centrifugation instructions and
send fresh plasma or send whole blood sample. It now becomes
vital that the specimen arrives in the lab within three hours of
collection.

Turnaround Time

Up to 72 hours

Test Includes

Factor IX Activity

Factor IX Ihibitor Titer

Pathology Interpretation

Preferred Volume

2 mL

Specimen Minimum Volume

1.5 mL

UCMC Collection Instructions

Specimens may be collected and sent to the laboratory at any
time.

Test can be added on to an existing order up to 4 hours
following the time of blood draw.

Additional Information

All 3.2% sodium citrate light blue top tubes require a ratio
of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL tube
requires 2.7 mL blood added to 0.3 mL of anticoagulant).

Reference Values

Factor IX: 80-147%

Factor IX Inhibitor: <0.6 Bethesda Units

DAY(S) AND TIME(S) PERFORMED

Factor (9) IX : Test performed Monday thru Friday from 8:00 am to 5:00
pm. Samples received in the lab after 5:00 pm will be run the
next weekday morning. Samples received on Friday after 5:00 pm
will be performed the following Monday. Please consult Lab medicine
resident on-call (pager 7910) if more rapid test performance is
deemed necessary.

Factor (9) IX Inhibitor: Samples received in the lab after 12:00 pm  (Monday-Thursday) will be run the next morning.              

Samples received after 12:00 pm on Friday will be run on Monday morning.

CPT

85250

85335

 

Reject Due To

Specimens that have wrong ratio of blood to anticoagulant
(fill volume) clotted or grossly hemolyzed.

Method Name

Clotting

Test Usage

The test is performed to measure Factor IX activity.

Bethesda assay for Factor IX
inhibitor titers generally performed only in the setting of
significantly decreased levels of Factor IX.

Critical Results

n/a

STAT DAY(S) AND TIME(S) PERFORMED

Not Available

STAT Turnaround Time

n/a

Fee Code

30585250

30485335

Synonyms

Factor IX, Factor 9 Assay, F9, Fa9, Factor 9 inhibitor Titer, Hemophilia B