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Test Code DOH Vitamin D, 25-Hydroxy, Total

Important Note

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

Specimen Type

Serum

Container/Tube

Red Top

Offsite Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be spun and
aliquoted
3. Transport refrigerated (2 – 8˚C)

Turnaround Time

4 hours

Test Includes

N/A

Preferred Volume

0.5 mL

UCMC Collection Instructions

1. Collect sample following standard collection methods
2. Within 2 hours of collection the sample must be received in
Laboratory Service Center
3. Transport room temperature (15 – 25˚C)

STAT Turnaround Time

N/A

Test Usage

Used in the assessment of vitamin D sufficiency in adults.

Critical Results

N/A

Test Limitations

Factors such as UV exposure, season, race ,and dietary intake
are all known to affect levels of 25-OH-D in humans. The
measurement of 25-OH-D is becoming increasingly important in the
management of patients with various disorders of calcium metabolism
associated with rickets, neonatal hypocalcemia, pregnancy,
nutritional and renal osteodystrophy, hypoparathyroidism, and
postmenopausal osteoporosis.

Fee Code

30082306

Synonyms

Vitamin D, 25-Hydroxy, 25-Hydroxy Vitamin

Stability: Room Temp

8 hours

Stability: Refrigerated

4 days

Stability: Frozen

24 weeks

Specimen Minimum Volume

0.2 mL

DAY(S) AND TIME(S) PERFORMED

24 hours, 7 days a week including holidays.

STAT DAY(S) AND TIME(S) PERFORMED

N/A

CPT

82306

Method Name

Electrochemiluminescence immunoassay (ECLIA)

Reference Values

Deficiency <12 ng/mL
Potential
Deficiency
12-19 ng/mL
Potential
Insufficiency
20-29 ng/mL
Optimum
Concentration
 ≥ 30
ng/mL

 

Reject Due To

Whole blood/unspun samples greater than 6 hours will be
rejected.

Additional Information

N/A

Included in Additional codes

N/A

Coverage Information

N/A