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HelpTest Code DOH Vitamin D, 25-Hydroxy
Specimen Type
Blood
Container
Red top
Preferred Volume
4 mL
Offsite Collection Instructions
Fasting (10 or more hours) is recommended but not required.
UCMC Collection Instructions
Fasting (10 or more hours) is recommended but not required.
Availability
Monday – Friday, one run per day
Turnaround Time
24 – 72 hrs
STAT Availability
Not Available
Test Usage
used in the assessment of vitamin D sufficiency
Test Methodology
radioimmunoassay
Additional Information
The measurement of 25-OH-D is becoming increasingly important in the management of patients with various disorders of calcium metabolism associated with rickets, neonatal hypocalcemia, pregnancy, nutritional and renal osteodystrophy, hypoparathyroidism, and postmenopausal osteoporosis
CPT Code
82306
Test Includes
quantitative determination of total 25-hydroxyvitamin D
Critical Results
None
Reference Range
10 – 52 ng/mL
Deficiency: <10 ng/mL
Borderline: 10 15 ng/mL
Winter: Mean +/ SD: 16 ± 7 ng/mL
Summer: Mean ± SD: 29 ± 12 ng/mL
Factors such as UV exposure, season, race ,and dietary intake are
all known to affect levels of 25-OH-D in humans.
Minimum Volume
2 mL
Fee Code
43110