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Test Code DIFF Differential Count

Specimen Type

Blood

Container

Lavender top, EDTA

Preferred Volume

4.5 mL

Availability

24/7

Turnaround Time

8 hours

STAT Availability

Available

UCMC Specimen Handling Instructions

Specimen must be received within 8 hours of collection. Refrigerate sample if transportation is delayed

Test Methodology

Wright stain light microscopy and automated cell counter (Sysmex XE5000)

CPT Code

85004

STAT Turnaround Time

1.5 hours

Minimum Volume

2 mL in 4 mL tube, 1.5 mL in 3 mL tube mL

Reference Range

 

 

Parameter Percentage (%) Absolute (K/uL)
Neutrophils/Granulocytes 39-75 1.12-6.72
Bands  0 - 6 0-0.66
Bands are counted on patients 6 years old and on all ED patients. On all other patients, "Left Shift" will be reported if Bands are 10% based on scan of the blood smear.
Lymphocytes 16-47 0.9-3.30
Monocytes  4 - 12 0.16-0.92
Eosinophils 0-7 0.0-0.6
Basophils 0-2 0.0-0.21
Immature Granulocyte <0.5 <0.04

 

 Reference ranges confirmed November, 2009

 

Critical Results

  • White Cell CT: <1.0 or >30.0 (x 10³/µL)
  • White Cell Differential: <0.5 Combined Neutrophil & Bands/uL when WBC is > 1.0 (x 10³/µL)
  • Any intracellular bacteria /yeast/ parasites present

Coverage Information

CMS has established a national coverage policy (NCD) for Medicare for this test as of November 25, 2002. Please document medical necessity. For more details click this link: https://www.cms.gov/medicare-coverage-database/details/ncd-details.aspx?NCDId=61&ncdver=1&bc=AgEAAAAAAAAA

Test Includes

Manual and Automated WBC Differentials include percentage and absolute number of White Blood Cell types, RBC morphology, WBC Morphology and platelet estimate. Performed manually or automated per laboratory protocol. To assure absolute values are reported, please order a CBC and a DIFF. Automated Differentials that are released without review by technologist only include percentage and absolute number of White Blood Cell Types.
 

The percentage of cells reported in a WBC Differential is based on counting 100 white blood cells. If the WBC is >15 K/ul, a 200 cell count is performed the percentage of each cell type counted is calculated (extrapolated to 100) and reported with the number of cells counted. When the WBC is very low (<1.5 K/ul) less than 100 cells are counted the percentage of each cell type counted is calculated (extrapolated to 100) and reported with the number of cells counted.
 

Only those cells seen during the count are reported. If the cell type is not reported, there were none of the cell type seen. For example, if 60% Granulocytes (Neutrophils), 25% Lymphocytes, 12% Monocytes, 2% Eosinophils, 1% Basophils are reported; then no other cells (reactive lymphs, metamyelocytes, myelocytes, promyelocytes, blasts) were seen.

Fee Code

53236

Synonyms

WBC Differential