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Test Code DDIM D-DIMER, Plasma

Specimen Type

Plasma

Container/Tube

Light blue top tube, 3.2% sodium citrate

Offsite Collection Instructions

Whole blood sample must arrive in the laboratory
within seven hours of specimen collection.

 

If transport time exceeds seven hours, follow
centrifugation instructions:

  • Samples must be centrifuged at >2500 x g for 10
    minutes at room temperature.
  • Carefully remove the plasma from the cells using a
    plastic transfer pipette avoiding the buffy coat.
  • Aliquot removed plasma into 2 plastic tubes
    containing a minimum of 500 uL of specimen in each tube.
  • The plasma samples must then be frozen at
    -20ºC.
  • Once solidly frozen, they must be shipped on dry
    ice.
    Samples that have thawed in transit will be rejected.

Note: For clients who do not have access to dry ice
DO NOT freeze the specimen. Follow the centrifugation instructions
and send fresh plasma or send whole blood sample. It now becomes
vital that the specimen arrives in the lab within seven hours of
collection. 

 

Turnaround Time

STAT: 60 minutes

Routine: 4 hours

Preferred Volume

1 mL

UCMC Collection Instructions

Specimens may be collected and sent to the laboratory at any
time.

 

Test can be added on to an existing order up to 8 hours
following the time of blood draw.

STAT Turnaround Time

1 hour

Additional Information

The literature cut-off value for exclusion of DVT/PE for
this
assay is 0.5 ug/mL Fibrinogen Equivalent Units (FEU).

http://www.accessdata.fda.gov/cdrh_docs/reviews/K162227.pdf

 

All 3.2% sodium citrate light blue top tubes require a ratio
of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL tube
requires 2.7 mL blood added to 0.3 mL of anticoagulant).

Fee Code

30585379

Test Usage

D-dimer are elevated in the setting of thrombosis. D-dimer
can be used to rule out DVT/PE. D-dimer are
also part of DIC scoring system

Critical Results

n/a

Specimen Minimum Volume

0.5 mL

DAY(S) AND TIME(S) PERFORMED

24/7

STAT DAY(S) AND TIME(S) PERFORMED

Available

CPT

85379

Method Name

Latex Immunoassay

Reference Values

<0.4 ug/mL FEU.

Reject Due To

Specimens that have wrong ratio of blood to anticoagulant
(fill volume) clotted or grossly hemolyzed.

Whole blood/unspun samples greater than 6 hours from collection time

will be rejected.