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Test Code CEBPA CEBPA Mutations, Gene Sequencing

Useful For

Initial evaluation of acute myeloid leukemia, both for assigning an appropriate diagnostic subclassification and as an aid for determining prognosis

Reporting Name

CEBPA Mutations, Sequencing

Specimen Type


Advisory Information

This test is intended for use at the time of diagnosis and not for disease monitoring.

Shipping Instructions

Specimen must arrive within 7 days (168 hours) of collection.

Necessary Information

The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date of collection

4. Specimen source

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Peripheral Blood

Container/Tube: EDTA (lavender top or ACD (yellow top)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

Specimen Stability: Ambient (preferred)/Refrigerate


Specimen Type: Bone marrow

Container/Tube: EDTA (lavender top) or ACD (yellow top)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

Specimen Stability: Ambient (preferred)/Refrigerate


Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions: Label specimen as extracted DNA from blood or bone marrow and provide indication of volume and concentration of the DNA.

Specimen Stability: Frozen (preferred)/Refrigerate/Ambient

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 7 days

Reject Due To


Mild OK; Gross reject






Paraffin embedded bone marrow aspirate clot, Bone marrow biopsies, slides or paraffin shavings, moderately to severely clotted

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81218-CEBPA (CCAAT/enhancer binding protein [C/EBP], alpha) (eg, acute myeloid leukemia), gene analysis, full gene sequence                                                    

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CEBPA CEBPA Mutations, Sequencing In Process


Result ID Test Result Name Result LOINC Value
MP019 Specimen: 31208-2
34634 Final Diagnosis: 34574-4

Reference Values

An interpretive report will be provided

Method Name

Mutation Detection in DNA Using Sanger Sequencing


1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (