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Test Code BC Bilirubin, Conjugated, Plasma

Specimen Type

Preferred: Plasma

Acceptable: Serum


Preferred: Lithium Heparin (Mint Green or Dark Green)

Acceptable: Serum (Red Top tube or Gold SST)


Preferred Volume

5 mL

Offsite Collection Instructions

1. Protect specimen from light.

2. Serum and plasma tubes should be centrifuged and aliquoted within 2 hours of collection.

3. Aliquoted samples could either be room temperature at 15-25°C or refrigerated at 2-8°C prior and during transportation.

4. If above criteria are not met, specimen will be rejected.

UCMC Collection Instructions

1. Protect specimen from light.

2. Samples need to be received in the lab within 2 hours of collection at room temperature.

3. Greater than 2 hours must be refrigerated at 2-8°C.

4. If the specimen cannot be sent to the lab within 6 hours of collection, it must be spun, aliquoted and refrigerated at 2-8°C.

5. All unspun specimens greater that 6 hours will be rejected.   

6. If above criteria are not met, specimen will be rejected.



Turnaround Time

4 hours

STAT Availability


Test Usage

Evaluation of jaundice and liver functions.

Test Methodology

Spectrophotometric, endpoint

Additional Information

Direct bilirubin levels must be assessed in conjunction with total and indirect levels and the clinical setting.

CPT Code


Test Includes

Quantitative determination of conjugated or direct bilirubin

Reference Range

Newborn to 14 days: 0.0 – 0.6 mg/dL  
> 14 days: 0.0 – 0.3 mg/dL

Critical Results


Test Limitations

Grossly hemolyzed specimens inhibit the diazo reaction and falsely low results may be seen.

STAT Turnaround Time

1 hour

Minimum Volume

2 mL

Fee Code


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