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Test Code B12 Vitamin B12

Important Note

This test may be affected by biotin interference. Patients on high dose biotin (5mg/day or more) should not have samples drawn until at least 24 hours after the last biotin administration.

Specimen Type

Plasma

Container/Tube

Li-Heparin Mint Green Top

Offsite Collection Instructions

1. Collect sample following standard collection methods, fasting
(8-14 hours) specimens is recommended.
2. Within 2 hours of collection the sample must be spun and
aliquoted
3. Transport refrigerated  (2 – 8˚C).

Turnaround Time

4 hours

Test Includes

N/A

Preferred Volume

0.5 mL

UCMC Collection Instructions

1. Collect sample following standard collection methods, fasting
(8-14 hours) specimens  is recommended.
2. Within 2 hours of collection the sample must be received in
Laboratory Service Center
3. Transport room temperature (15 – 25˚C).

STAT Turnaround Time

N/A

Test Usage

Useful as an aid in the investigation of macrocytic anemia and
workup of deficiencies seen in megaloblastic anemias.

Critical Results

N/A

Test Limitations

A normal serum concentration of vitamin B12 does not rule out
tissue deficiency of vitamin B12. The most sensitive test for
vitamin B12 deficiency at the cellular level is the assay for
MMA.

If clinical symptoms suggest deficiency, measurement of MMA and
homocysteine should be considered, even if serum vitamin B12
concentrations are normal.

Fee Code

30182607

Synonyms

Cyanocobalamin, B12

Stability: Room Temp

2 hours

Stability: Refrigerated

48 hours

Stability: Frozen

24 hours

Specimen Minimum Volume

0.2 mL

DAY(S) AND TIME(S) PERFORMED

24 hours, 7 days a week including holidays.

STAT DAY(S) AND TIME(S) PERFORMED

N/A

CPT

82607

Method Name

Electrochemiluminescence Immunoassay (ECLIA)

Reference Values

240 – 900 pg/mL

Reject Due To

Whole blood/unspun specimens greater than 6 hours will be
rejected.

Additional Information

N/A

Included in Additional codes

N/A

Coverage Information

N/A