Sign in →

Test Code AFBIL Bilirubin, Amniotic Fluid

Important Note

Use Sunquest code BILIAS to order

Reporting Name

Bilirubin, AF

Useful For

Evaluation of Rh disease, ie, hemolytic disease of the fetus


Monitoring disease progression to assess need for fetal transfusion

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Amniotic Fld

Necessary Information

Gestational age at time of amniocentesis is required.

Specimen Required

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Amniotic fluid container

Submission Container/Tube: Opaque, amber vial (T192)

Specimen Volume: 3.5 mL

Collection Instructions:

1. Centrifuge, separate supernatant, and send both supernatant and sediment.

2. Protect specimen from light.

3. Label specimens as sediment and supernatant.

UChicago Medicine Medical Laboratories Note:

Offsite Collection Instruction:


Protect from light, wrap in foil or dark paper


Specimen Minimum Volume

1.7 mL

Specimen Stability Information

Specimen Type Temperature Time
Amniotic Fld Frozen 70 days

Reference Values

Interpretation of fetal risk is dependent upon gestational age.

Refer to either the Queenan Curve (gestational age <27 weeks) or the Liley Chart (gestational age >27 weeks) listed under Interpretation of Amniotic Fluid Bilirubin Results (Delta OD 450) in Special Instructions.

Day(s) and Time(s) Performed

Monday through Saturday; Continuously

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
AFBIL Bilirubin, AF 12476-8


Result ID Test Result Name Result LOINC Value
AMBL Amniotic Fluid 12476-8
AFCMT Other Information 48767-8
GSTN Gestation 18185-9

Reject Due To






Mild OK; Gross OK



Method Name

Spectrophotometric Scan