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Test Code ACST Acanthamoeba Stain

Specimen Type

Corneal scraping, Eye, Contact lens solution and case


Glass slides obtained from the Microbiology lab (2-6133)

Offsite Collection Instructions

See UCMC Collection Instructions

UCMC Collection Instructions

Specimen Source: Corneal scraping
Specimen Container: Glass slides obtained from the Microbiology lab. Please call the lab at 773-702-6133.
Specimen Collection: Specimen collected by ophthalmologist using a sterile spatula to scrape ulcers and/or lesions. Scrapings are directly inoculated onto glass media (between the frosted ring). Please label the frosted part of the slide with patient information.
Specimen Transport: 24 hr, no refrigeration


Specimen Source: Contact lenses, solution, and case
Specimen Collection: Send contact lenses, contact cases, and/or contact solution to Microbiology lab.
Specimen Transport:  24 hr, no refrigeration

See Microbiology Specimen Collection:

  • Category “Eye” in Microbiology Specimen Collection Tables, Table 1 “Bacteriology, Chlamydia, Mycobacteriology (AFB), Mycology, Virology”


Monday through Saturday

Turnaround Time

Same day if received in Microbiology lab by 2:00 p.m. Monday through Saturday.

STAT Availability

Not Available

Test Methodology

Fluorescent stain (Acridine Orange Stain)

CPT Code


Reference Range


Fee Code