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Test Code HBSAG Hepatitis B Surface Antigen, Serum

Biohazard Infectious

Specimen Type

Serum

Container

Red Top tube or Gold STT

Preferred Volume

5 mL

Offsite Collection Instructions

1. Serum tubes should be centrifuged and aliquoted within 2 hours of collection.

2. Aliquoted samples should be refrigerated at 2-8°C prior and during transportation.

3. If above criteria are not met, specimen will be rejected.

4. Li-Heparin tubes will be rejected.

UCMC Collection Instructions

1. Samples need to be received in the lab within 2 hours of collection at room temperature.

2. Greater than 2 hours must be refrigerated at 2-8°C.

3. Greater than 6 hours refrigerated specimens must be centrifuged and aliquoted.

4. If above criteria are not met, specimen will be rejected.

5. Li-Heparin tubes will be rejected.

 

Note: Patients on heparin therapy or specimens drawn from heparinized lines will be rejected.

Availability

24/7

Turnaround Time

4 hours

STAT Availability

Not Available

Test Usage

The assay may be used in conjunction with other serological and clinical information to diagnose individuals with acute or chronic hepatitis B infection.

 

The assay may also be used to screen for hepatitis B infection in pregnant women to identify neonates who are at risk of acquiring hepatitis B during the perinatal period.

Test Methodology

Electrochemiluminescence Immunoassay (ECLIA)

CPT Code

87340

Critical Results

None

Reference Range

Non-reactive

 

Reactive*
 

A nonreactive test result does not exclude the possibility of exposure to or infection with hepatitis B virus. A nonreactive test result in individuals with prior exposure to hepatitis B may be due to antigen levels below the detection limit of this assay or lack of antigen reactivity to the antibodies in this assay.
 

*NOTE: Reactive specimens subject to confirmatory testing by Hepatitis B Surface Antigen Neutralization assay. High level reactivity does not require confirmation by supplemental Hepatitis B Surface Antigen Neutralization assay.

Test Limitations

It has been reported that certain assays will not detect all HBV mutants.  If acute or chronic HBV infection is suspected and the HBsAg result is nonreactive it is recommended that other HBV serological markers be tested to confirm the HBsAg nonreactivity.
 

The performance of the assay has not been established for populations of immunocompromised or immunosuppressed patients.

Minimum Volume

1 mL

Fee Code

69201

Test Includes

Qualitative detection of hepatitis B surface antigen (HBsAg) in human serum