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Test Code GPT Alanine Aminotransferase, Plasma

Specimen Type

Preferred: Plasma

Acceptable: Serum


Preferred: Lithium Heparin (Mint Green or Dark Green)

Acceptable: Serum Red Top Tube or Gold SST

Preferred Volume

5 mL

Offsite Collection Instructions

1. Serum and plasma tubes should be centrifuged and aliquoted within 2 hours of collection.

2. Aliquoted samples could either be room temperature at 15-25°C or refrigerated at  2-8°C prior and during transportation.

3. If above criteria are not met, specimen will be rejected.



Turnaround Time

4 hours

STAT Availability


Test Usage

ALT is useful in the diagnosis and monitoring of liver disease.   Serum elevations of ALT activities are rarely observed except in parenchymal liver disease, since ALT is a more liver-specific enzyme than AST.  Elevated levels of the transaminases can indicate myocardial infarction, hepatic disease, muscular dystrophy, and organ damage.

Test Methodology

Spectrophotometric, kinetic

CPT Code


Test Includes

Quantitative determination of alanine aminotransferase (ALT) in plasma

Reference Range

8-35 IU/L

Critical Results


Test Limitations

Isoniazid can cause artificially low and Furosemide artificially high alanine aminotransferase results at therapeutic concentrations.
Cyanokit (hydroxocobalamin) at therapeutic levels causes significantly lower results.

STAT Turnaround Time

1 hour

Fee Code




Minimum Volume

2 mL

UCMC Collection Instruction

1. Samples need to be received in the lab within 2 hours of collection at room temperature.

2. Greater than 2 hours must be refrigerated at 2-8°C.

3. If the specimen cannot be sent to the lab within 6 hours of collection, it must be spun, aliquoted and refrigerated at 2-8°C.

4. All unspun specimens greater than 6 hours will be rejected.  

5. If above criteria are not met, specimen will be rejected.