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Test Code FA9 Factor IX (9) Assay

Specimen Type



Light blue top tube, 3.2% sodium citrate

Preferred Volume

2.7 mL

Offsite Collection Instructions


  • Samples must be centrifuged at >1500 x g for 10 minutes at room temperature.
  • Carefully remove the plasma from the cells using a plastic transfer pipette avoiding the buffy coat.
  • Aliquot removed plasma into 2 plastic tubes containing a minimum of 500 uL of specimen in each tube.
  • The plasma samples must then be frozen at -20ºC.
  • Once solidly frozen they must be shipped on dry ice. Samples that have thawed in transit will be rejected.
  • Note: For clients who do not have access to dry ice do not freeze the specimen. Follow the centrifugation instructions or send the whole blood sample. It now becomes vital that the specimen arrives in the lab within three hours of collection.

UCMC Collection Instructions

Specimens may be collected and sent to the laboratory at any time.

Test can be added on to an existing order up to 4 hours following the time of blood draw.


Test performed 7 days a week from 8:00 am to 10:30 pm. Samples received in the lab after 10:30 pm will be run the next morning.

Turnaround Time

8 hours

STAT Availability

Not Available

Test Usage

The test is used to measure Factor IX activity.

Test Methodology


Additional Information

All 3.2% sodium citrate light blue top tubes require a ratio of 9 parts blood to 1 part anticoagulant (example: a 3.0 mL tube requires 2.7 mL blood added to 0.3 mL of anticoagulant).

CPT Code


Test Includes

Factor IX Activity

Reference Range

80-147%, effective 7/27/05

Minimum Volume

2.7 mL


For pediatric patients = 1.8 mL

Fee Code



Factor IX (9) Coagulant Assay